Specimen Preparation Protocols

These specimen preparation protocols are from work done in the E.M. Lab for Dartmouth faculty.

Some of the abbreviations not listed on the pages are as follows:
LC = lead citrate; cpd = critical point drying; UA = uranyl acetate.

If you use these protocols and have questions, feel free to contact us .

In addition to the protocols listed here, there are also some editable pages where we encourage you to post and update your own protocols.For an access ID:password pair, contact E. M. Facility staff.

E. M. Specimen Preparation Wiki

New protocols will be listed in the Wiki - updated 11-02-12.

SEM Protocol

• Cartilage cryofracture
• Fibroblast Cells grown on (in-house) CHITIN matrix
• Kidney Tubule Cells from Mice
• Acetolysis/SEM Prep of Pollen
• Pseudomonas
• Saccharopolyspora spinosa

TEM protocols

• Clostridia thermicellum
• Culture cells mitotic apparatus - TEM/IEM Flat Embedding and Serial Section
• Drosophila eye
• Growth plate vasculated interface of 6 week old mice
• Mouse embryonic heart
• Soybean Root Nodules
• Tissue Culture Cells (Mast cells)
• Tissue Culture Cells (monocytes and dendritic cells)
• Toxoplasma
• Preparation of yeast cells for immuno-electron microscopy.

Other Methods

• Poly-L-Lysine Coated Coverslips.
• Preparation of indirect carbon films.
• Frozen Section protocol for cryo UltraMicrtome.

Last modified on 06/18/01.