arabidopsis

Jack Laboratory

Dartmouth College, Department of Biological Sciences

Hanover, NH 03755

Thomas.P.Jack@Dartmouth.EDU

 

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Enhancer Traps in Arabidopsis

PI alleles

Potential Thesis Projects

Publications

Department of Biological Sciences

Molecular and Cellular Biology Graduate Program

Dartmouth College

 

Sequence of AP3 alleles

Allele

Mutagen

Strength

Background

Mutation

Source / Reference

 

 

 

 

 

 

ap3-1

EMS

intermediate
temp. sens.

Ler

K153M
5' splice site intron 5

1-4

ap3-3

EMS

strong

Ler

Q18--> stop

2

ap3-4

EMS

strong

Ler

Q7-->stop

2

ap3-5

EMS

strong

Ler

Q83-->stop

2

ap3-6

EMS

strong

Col

A32-->P

Roger Innes-ED1503

ap3-7

EMS

strong

Col

5' splice site intron 4: GT-->GA

George Redei

ap3-8

EMS

strong

ap2-1 (Ler)

3' splice site intron 1: AG-->AA

Zhongchi Liu-ZL231

ap3-9

EMS

strong

Ler

A32-->V

John Alvarez-S1357

ap3-10

EMS

strong

Ler

A32-->P

John Alvarez-S1359

ap3-11

EMS

weak

ap3-1 - Ler

intragenic suppressor ap3-1
T-->A - intron 4 - 33 bp from the 3' end of the intron - branchpoint?

5

ap3-12

EMS

strong

Ler

P60-->S

Hongjun Xiang-650E1

ap3-13

T-DNA

strong

WS

T-DNA inserted in intron 6

Ioan Negrutiu- DJY12

 

 

 

 

 

 

References

1. Bowman, J. L., Smyth, D. R. and Meyerowitz, E. M. (1989). Genes directing flower development in Arabidopsis. Plant Cell 1, 37-52.
2. Jack, T., Brockman, L. L. and Meyerowitz, E. M. (1992). The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell 68, 683-697.
3. Koornneef, M., van Elden, J., Hanhart, C. J., Stam, P., Braaksma, F. J. and Feenstra, W. J. (1983). Linkage map of Arabidopsis thaliana. J. Hered. 74, 265-272.
4. Sablowski, R. and Meyerowitz, E. M. (1998). Temperature-sensitive splicing in the floral homeotic mutant apetala3-1. Plant Cell 10, 1453-1463.
5. Yi, Y. and Jack, T. (1998). An intragenic suppressor of the Arabidopsis floral organ identity mutant apetala3-1 functions by suppressing defects in splicing. Plant Cell 10, 1465-1477.