Research Associate Professor and Director of the Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center
Dr. Givan received an A.B. degree from Bryn Mawr College in 1963 and a Ph.D. in Biology from Harvard University in 1968. She did post-doctoral research in the Department of Biochemistry and Biophysics at the University of California at Davis. In 1971, she moved to England where she held appointments as a Research Associate in the Department of Plant Biology at the University of Newcastle upon Tyne, as a Course Tutor and Counsellor at the Open University, and then, from 1985 to 1991, as a Senior Scientific Officer in the Flow Cytometry Facility connected with the Department of Surgery of Newcastle University Medical School. Dr. Givan joined the Department of Physiology at Dartmouth in 1992 and is now a Research Associate Professor and is Director of the Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center.
Dr. Givan's principal research interests concern the development and use of flow cytometric and image analysis methods for quantitating the changes and responses of leukocytes to immunological stimuli. As Director of the Englert Cell Analysis Laboratory, she is involved with training students and scientists in the use of flow and imaging instrumentation and collaborates with research groups throughout the Medical School and College on projects that involve these techniques.
Givan, A.L., Fisher, J.L., Waugh, M., Ernstoff, M.S., Wallace, P.K. (1999). A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens. J. Immunol. Methods 230:99-112.
Langweiler, M. and Givan, A.L. (2000). Flow Cytometry and Quality Control : An Uneasy Alliance. Chapter in Immunophenotyping (JKA Nicholson and CC Stewart, eds). John Wiley & Sons, Inc., NY, pp 23-47.
Givan, A.L.(2000). The Basics of Staining for Cell Surface Proteins. Chapter in In Living Color:Flow Cytometry and Cell Sorting Protocols (R Diamond and S DeMaggio, eds). Springer Verlag, Berlin, pp 142-164.
Benbow, U., Orndorff, K.A., Brinckerhoff, C.E., Givan, A.L. (2000). A confocal assay for invasion: The use of propidium iodide fluorescence and laser reflectance to determine the rate of migration of cells through a matrix. Cytometry 40: 253-259.
Givan, A.L. (2001). Principles of Flow Cytometry: An Overview. Chapter in Cytometry (Z. Darzynkiewicz, ed), Academic Press, San Diego, pp 19-50.
Givan, A.L. (2001). Flow Cytometry: First Principles. Second Edition. John Wiley and Sons, Inc., New York.
Rassias, A.J., Givan, A.L., Marrin, C.A.S., Whalen, K., Pahl, J., Yeager, M.P. (2002). Insulin increases neutrophil count and phagocytic capacity after cardiac surgery. Anesthesia and Analgesia 94: 1113-1119.
Bercovici, N., Givan, A.L., Waugh, M.G., Fisher, J.L., Vernel-Pauillac, F., Ernstoff, M.S., Abastado, J-P., Wallace, P.K. (2003). Multiparameter precursor analysis of T-cell responses to antigen. J. Immunol. Methods 276: 5-17.
Givan, A.L. (2004). Flow Cytometry: An Introduction. Chapter in Flow Cytometry Protocols, 2nd edition (T.S. Hawley, R.G. Hawley, eds.). Methods in Mol. Biology, vol. 263. Humana Press Inc., Totowa, NJ, pp. 1-31.
Givan,A.L., Fisher, J.L.,Waugh, M.G., Bercovici, N., and Wallace, P.K. (2004). Use of Cell-Tracking Dyes to Determine Proliferation Precursor Frequencies of Antigen-Specific T Cells. Chapter in Flow Cytometry Protocols, 2nd edition (T.S. Hawley, R.G. Hawley, eds.). Methods in Mol. Biology, vol. 263. Humana Press Inc., Totowa, NJ, pp. 109-124.[Last updated 8/9/05]
