- Primers for 1kb Flanks
Here are the statistics for this primer design run:
155 failed to design primers on both sides.
Of these, 129 did not have flanking issues (they failed for other reasons), and 3 could not find sufficient flanking on either side. The other 23 could not find sufficient flanking to make primers on one side only.
418 failed on only one side. Of these, 405 did not have flanking issues (they failed for other reasons). The other 13 could not find sufficient flanking to make primers on one side only.
10047 successfully made both primer pairs.
Thus the overall sucess rate was (10047*2+418)/(10620*2-3*2-23-13) = 96.7638%
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