Molecular Biology & Proteomics Core Facilty Dartmouth College

Protein Sequencing (Edman)

Edman sequencing is the best way to determine the N-terminal amino acid of a protein or peptide. It is an excellent tool for confirming recombinant protein's N-termini (reading frame) and to confirm the sequence of synthetic peptides. Although most protein identification is more easily done using mass analysis techniques, Edman sequencing is still useful for sequencing “unknown” proteins from any species if large enough quantities are available. It is also useful for the determination of phosphorylation sites in isolated labeled peptides. The MB&P Core has an ABI Model 494 protein sequencer; this instrument is an integral part of our protein services. The Model 494 is capable of N-terminal sequencing at the 10 picomole level (500 nanograms of a 50,000Da protein).

General Information

Sample preparation [HTML] [PDF download]

Protein Services Sample Order Form

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				Protein Sequencing (Edman) Edman sequencing is the best way to determine the N-terminal amino acid of a protein or peptide. It is an excellent tool for confirming recombinant protein's N-termini (reading frame) and to confirm the sequence of synthetic peptides. Although most protein identification is more easily done using mass analysis techniques, Edman sequencing is still useful for sequencing “unknown” proteins from any species if large enough quantities are available. It is also useful for the determination of phosphorylation sites in isolated labeled peptides. The MB&P Core has an ABI Model 494 protein sequencer; this instrument is an integral part of our protein services. The Model 494 is capable of N-terminal sequencing at the 10 picomole level (500 nanograms of a 50,000Da protein). General Information Sample preparation [HTML] [PDF download] Protein Services Sample Order Form Contact


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