Protein Expression Analysis

The MB&P Core operates a Thermo Finnigan LTQ-XL ion trap mass spectrometer and an ABI Voyager DE Pro MALDI-TOF mass spectrometer. The LTQ-XL is the most useful instrument for proteomics work and is the basis of our protein identification service. The LTQ is equipped with an Eksigent nanoflow HPLC for performing LC/MS/MS for protein identification projects. The Sequest database search engine is available in the Core Facility. The Mascot search engine is site licensed and available campus-wide. The Voyager is available as a walk up instrument. The Core Facility holds regular training classes for those interested in using the MALDI-TOF mass spectrometer.

Protein Identification

Submission of Coomassi Stained gel Slice for Protein Identification

Submission of Silver Stained gel Slice for Protein Identification

Protocol for Reduction and Alkylation prior to Gel Electrophoresis of Samples Submitted for Protein Identification

Guidelines for Submission of Purified Whole Protein Samples for MALDI & ESI MS

Protein Services Order Form

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