The mission of the Dartmouth College Molecular Biology Core Facility is to provide products, services andsupport to help investigators solve basic and clinical research problems related to macromolecules (DNA,RNA, proteins) of biomedical interest. It is our goal to offer the highest quality products and servicespossible at reasonable prices. The charges to investigators will never be more than what they cost thefacility for time and materials. In some instances, for example oligonucleotide synthesis, we will notalways be able to directly compete with outside providers based on price alone. However, we also offerother services, such as DNA sequencing, which are not generally commercially available or are notcost-effective for the researcher to use. In addition to competitive pricing, what the MB core can offerthat commercial services cannot is to provide a higher quality product or service, provide rapid access andturn-around time, back all services up with a "money-back" guarantee, and to provide detailed and expertadvice and support to the research community. The overall goal of the facility is to become a valuable andresponsive in-house resource for helping investigators obtain their research objectives involvingmacromolecular sequencing and synthesis.

The current services we offer are DNA sequencing, protein sequencing, and DNA synthesis. The facility is equipped with: a model 476A protein sequencer, a Model 3100 DNA sequencer, Model 7700 Sequence Detector and two a model 394/5 DNA synthesizers all manufactured by Applied Biosystems Inc. Two Hewlett Packard HPLCs (a model 1050 and a model 1090) are also part of the facility's current equipment. We have recently expanded our services in Protein analysis. We now have a ThermoQuest LCQ Deca XP LC-Mass Spectrometer and an ABI Voyager DE Pro. We have also updated our Edman sequencing capabilities with purchased an ABI Model 494 Procise sequencer. We offer a wide variety of protein related services see our price sheet for details. The Molecular Biology Core Facility has taken over management of the Molecular Dynamics phosphorimaging systems and the X-ray film processors at the Hanover and Borwell sites. All services are performed on first come - first serve basis unless otherwise noted in the individual service protocols. Charges for services will be made directly to investigator accounts.

The MB Core currently has four full-time staff members: Stephen Bobin, who is the Core Manager, Christian Lytle DNA Services, Susan Kennedy Protein Services and Sharon Tyndall Administrative assistant. The Core facility itself is open 7:30 AM to 5 PM Monday through Friday and is also available by BlitzMail and FAX. There is a folder on the Dartmouth College Public servermaintained by the facility where software, protocols and other documents can be obtained. The Core is fiscally managed by theNorris Cotton Cancer Center as a shared resource. The Director of this resource is Joshua Hamilton, whooversees the operation of the core. The Director also serves as Chair for a faculty MB Core Advisory Committee which meets quarterly and reports to the NCCC Executive Committee. In addition to thecharge-back system for services, the budget for the MB Core is currently underwritten by funds from theNCCC Core Grant, the Dartmouth Superfund Program Project, and funds from Dartmouth Collegeadministered through the Molecular Genetics and Structural Biology groups.

To reach our files on PUBLIC:

Open PUBLIC

Campus Info and Organizations

Academic Organizations

Molecular Biology Core Facility