Department of Biochemistry
Geisel School of Medicine at Dartmouth
7200 Vail Building, Rm 408A
Hanover, NH 03755-3844 USA
Areas of Expertise: Structural biology; protein-protein interactions; CFTR trafficking regulators
The goal of research in the Madden lab is to understand the functional characteristics of ion channels in terms of their molecular structure and interactions. Dean's group investigates the regulation of ion channel activity through interactions with PDZ proteins that guide intracellular trafficking, uptake, and recycling. Our particular focus is on CFTR, the chloride channel mutated in patients with cystic fibrosis. We use biophysical techniques to study both endogenous (human) and exogenous (bacterial) proteins that destabilize CFTR, leading to a breakdown of antibacterial defenses in the lung. In addition, we use electron microscopy and X-ray crystallography to study conformational changes in glutamate receptor ion channels that are responsible for triggering and controlling post-synaptic neuronal signals.