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Microscope diagram
| Diagram of the optical and equipment setup necessary for AVEC-DIC microscopy.
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Smooth ER vesicles
| This images was taken using video-enhanced DIC microscopy. Images on the right were taken with classic Hamamatsu real-time analogue video processing equipment. Images on the left were digitally aquired and digitally processed using an Intel-based CPU and Metamorph software. Top to bottom: no processing, background subtract, contract enhancement.
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Actin from Clam Egg extract
| Filaments of actin stained with fluorescently-tagged antibodies |
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ImmunoEM
| Vesicle fraction from squid axoplasmic cells were subjected to gold-bead-conjugated kinesin or myosin V proteins. Small beads are kinesin, larger beads are myosinV
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Fluorescently stained ER vesicles
| Vesicles from extruded axoplasm were stained with the fluorescently labeled ER-specific dye, DiOC6
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Antibody motility inhibition
| An antibody against a short amino acid sequence in the tail of Myosin V was used to inhibit vesicle motility on actin filaments. Kinesin-specific tubulin motility was unaffected
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Tubulin and actin fluorescence
| Tubulin and actin filaments were stained to localize the tracks that the motile filaments follow. In this way one can determine whether kinesin or MyosinV is the predominant motor
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EM of TEVO
| Electron micrograph of endoplasmic reticulum vesicles showing the extensive filaments
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Protein alignment
| Alignment of Myosin V proteins from several eukaryotic species
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Video-enhanced light microscopy
