Transgenic and Genetic Construct Shared Resource
Leadership
Steven Fiering, PhD, director
Jen Fields, BS, technician
Laurie Horne, technician
Sandy Warner, technician
Mission Statement
Our mission as part of the Dartmouth Immunology COBRE is to provide support for the generation and experimental utilization of transgenic animals for immunology experiments by COBRE project leaders and the general immunology research community.
Case Study
Recent work with the lab of Jose Conejo-Garcia has generated a novel transgenic mouse model of ovarian cancer that can be initiated by surgically injecting adenovirus expressing Cre into the ovarian bursa. The model utilizes mice that are homozygous for "floxed" p53 and hemizygous for lox.stop/lox mutant KRAS. These animals rapidly develop ovarian cancer after the adenovirus cre exposure and by 60 days after such exposure the ovarian tumor is large and the experiment must be stopped. Dr. Conejo-Garcia is studying the early events in ovarian cancer development and techniques for early detection of cancer using this model.
List of Services
Transgenic Mouse generation
- ES cell culture and genetic manipulation
- Generation of ES cell chimeric mice to make transgenic mice
- Oocyte injection with DNA to make transgenic mice
Transgenic mouse maintenance
- Biopsies, genotyping, tagging and general breeding and husbandry
- Rederivation of pathogen infected mice being imported to Dartmouth
- Preservation of transgenics lines by freezing
Genetic construct services
- Generation of small genetic constructs by recombineering in yeast
- Generation of bacterial artificial chromosome genetic constructs by recombineering in E. coli
- Generation of mice with a humanized immune system
Contact
Steve Fiering, 603-653-9966
Fiering@dartmouth.edu
Jen Fields, 603-6035
Jen.Fields@dartmouth.edu