Scott A. Gerber, Ph.D.

Assistant Professor of Genetics

Quantitative Mass Spectrometry and Proteomics

Mass spectrometry has become a cornerstone technology for the field of proteomics.  The analysis of proteins and protein post-translational modifications is accomplished by the powerful combination of MS, microcapillary liquid chromatography, and computer-assisted sequence database searching.  This mixture of maturing technologies provides a scientist with the ability to elucidate protein-protein relationships, characterize functionally descriptive protein post-translational modifications, and discover new protein isoforms or splice variants, even from limiting sample amounts.  In addition, we and others have recently developed novel mass spectrometry-based methods that extend the capability of routine protein identification to quantitative analyses as well.  When coupled with state-of-the-art instrumentation, the differential display of dynamic changes of protein expression, modification, or localization by MS becomes practical.

Publications

• Gerber S.A., Scott CR, Turecek F, and Gelb MH, Analysis of rates of multiple enzymes in cell lysates by electrospray ionization mass spectrometry, J. Am. Chem. Soc.  v121, pp 1102-3, 1999.
• Gygi SP, Rist B, Gerber S.A., Turecek F, Gelb MH, and Aebersold R, Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.Nat Biotechnol v17, pp 994-9, 1999.
• Gerber S.A., Scott CR, Turecek F, and Gelb MH, Direct profiling of multiple enzyme activities in human cell lysates by affinity chromatography/electrospray ionization mass spectrometry: application to clinical enzymology. Anal Chem v73, pp 1651-7 2001.
• Gerber S.A., Rush J, Stemmann O, Kirschner MW, and Gygi SP, Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS.Proc Natl Acad Sci U S A v100, pp 6940-5, 2003.

Scott A. Gerber, Ph.D.
Assistant Professor of Genetics
Member Norris Cotton Cancer Center
Dartmouth-Hitchcock Medical Center
Rubin Building
Lebanon, New Hampshire 03756
603 653 3679
Scott.A.Gerber@Dartmouth.EDU