Clinical Procedure Manual

Table of Contents

Acquisition and Preparation of Tissue for Electron Microscope Examination

All of the following procedures are to be performed in a hood or a well vented tissue processor.

• Solid Tissue Surgical Specimen
• Protocol for Muscle Biopsy
• Protocol for Nerve Biopsy
• Protocol for Buffy Coat
• Nasal Scrapings for Cilia , Aspirate Fixation and Preparation for Bone Marrows and Other Single Cell Preparations.
• Aspirate Fixation and Preparation for Bone Marrows and
  Other Single Cell Preparations.
• Directions for Preparation of E.M. Tissue Other Than
  From Dartmouth-Hitchcock Medical Center
• Light Microscopy Section Preparation
• Electron Microscopy Section Preparation
• Staining of 1200 Å Sections on Grids With Heavy Metals for Electron Microscope Section Staining
• Protocol for SEM /X-ray Analysis of Tissue
  Formulas for Solutions
• Formulas for Solutions
  • Buffer Formulas
  • Fixative Formulas
  • Post Fixatives
• Staining Formulas
• Embedding Media
• References

reviewed 01-24-07 CPD