Skip to main content

EHS Training

EHS has completed the new on line Introduction to Laboratory Safety Course, this course replaces the monthly classroom offerings and removes any remaining barriers to full compliance by anyone working in a DC laboratory specifically undergraduates with class conflicts.


Introduction to Laboratory Safety on line course


Radiation Safety Classroom Training - Contact EHS 646-1762

Working with rDNA

BSL-2 Exposure Control Plan


The use of Recombinant DNA Technology (rDNA) in the laboratory is highly regulated by the National Institutes of Health (NIH). Strict adherence to the NIH Guidelines For Research Involving Recombinant Or Synthetic Nucleic Acid Molecules is essential to maintain the proper regulatory oversight of rDNA at Dartmouth College.

Oversight and Approvals of Research

All rDNA research is required to have the approval of the Dartmouth Institutional Biosafety Committee (IBC), PRIOR to commencement of research.

Refer here for more information regarding the oversight and approval of research by the IBC.

Dartmouth College currently uses the online compliance dashboard, BioRAFT, to track, maintain compliance, and issue specific personnel trainings as it pertains to rDNA usage in the laboratory. For help using BioRAFT to input your research information for institutional review, please refer to the following link. (coming soon)

Regulations regarding the roles and responsibilities of rDNA usage in the laboratory are conveyed in the NIH Guidelines.

The responsibility of the Primary Investigator (PI), according to the NIH Guidelines is as follows:

Section IV-B-7. Principal Investigator (PI)

On behalf of the institution, the Principal Investigator is responsible for full compliance with the NIH Guidelines in the conduct of recombinant or synthetic nucleic acid molecule research…….The Principal Investigator is responsible for ensuring that the reporting requirements are fulfilled and will be held accountable for any reporting lapses.


Please refer to the following in reference to the Roles and Responsibilities of the PI as it refers to rDNA research.

Investigator Responsibilities (NIH Guidelines)

NIH brochure pertaining to Investigator Responsibilities

Important References for rDNA Researchers

Oversight and approval requirements for transgenic animals

Working with Viral Vectors

Vaccinia Use and Surveillance Policy

Working Safely with Lentiviral Vectors

Working Safely with Adenoviral Vectors

Last Updated: 7/19/13