(This shows with PI/7-AAF and fixable viability dyes)
(Includes PI/7-AAD and Fixable Dyes. eBioscience Specific)
Cell surface staining:
Intracellular and Intranuclear Staining:
(covers a couple eFluor dyes as well as CFSE; should double check that the CFSE concentration is consistent with our SOP)
How many events are enough?
Minimum information about a flow cytometry experiment
BD fluorescence spectrum viewer
Amine-reactive dyes for dead cell discrimination in fixed samples (Perfetto et al., 2010)
BD compensation multicolor technical bulletin (2009)
Optimizing a multi-colour immunophenotyping assay (Mahnke & Roederer, 2007)
A very helpful tool to appreciate absolute cell numbers to be expected is the Frequencies of Cell Types chart published by StemCell Technologies.