Immune Monitoring Laboratory at Dartmouth-Hitchcock Medical Center

News

CMS - 1.6.8 - Pouebo

Useful Links

Cell Preparation:

http://media.ebioscience.com/data/pdf/best-protocols/cell-preparation-for-flow-cytometry.pdf

Annexin V:
(This shows with PI/7-AAF and fixable viability dyes)
Viability Staining:
(Includes PI/7-AAD and Fixable Dyes. eBioscience Specific)
Cell surface staining:
Intracellular and Intranuclear Staining:
Proliferation:
(covers a couple eFluor dyes as well as CFSE; should double check that the CFSE concentration is consistent with our SOP)

http://www.maeckerlab.typepad.com/

http://www.isac-net.org/

How many events are enough?

Minimum information about a flow cytometry experiment (MiFlowCyt)

Minimal Information About T cell Assays (MIATA)

MIATA checklist

MIATA Easy Reporting of Experiments

Macrophage activation and polarization: nomenclature and experimental guidelines

BD fluorescence spectrum viewer

Amine-reactive dyes for dead cell discrimination in fixed samples (Perfetto et al., 2010)

BD compensation multicolor technical bulletin (2009)

Optimizing a multi-color immunophenotyping assay (Mahnke & Roederer, 2007)

A very helpful tool to appreciate absolute cell numbers to be expected is the Frequencies of Cell Types chart published by StemCell Technologies.