autoMACS/Robosep

autoMACS

The autoMACS Separator (Miltenyi Biotec) is a benchtop instrument for high-speed automated cell enrichment. Employing MACS Technology, the autoMACS Separator is designed for positive selection as well as depletion of magnetically labeled cells.

Using the autoMACS Separator is simple: add the magnetic labeleing reagent to a cell suspension and choose an application program from the autoMACS touchscreen. In just a few minutes, cells are magnetically separated and ready for cell analysis or further flow cytometric cell sorting. Rare cells can be enriched up to 10,000-fold, or unwanted cells are depleted up to 99%.

MACS magnetic cell sorting does not alter the flow cytometric or functional properties of cells. For specific magnetic labeling a wide range of MACS microbeads and MACS antibodies is available.

  • Versatile -compatible with more than 200 MACS cell separation reagents for the isolation of virtually any cell type from any species, even from whole blood.
  • Simple -touchscreen interface and preset programs for optimal results, ready-to-use MACS buffers for easy operation.
  • Speed -high-speed sorting of more than 10 million cells per second.
  • Column capacity -2x108magnetically labeled cells; 4x109 total nucleated cells.
  • Input volume -from 0.2 ml to 50 ml.
  • Compatible -perfect companion for flow cytometry, no MicroBead removal required for further experiments.

Cell isolation kits for use with autoMACS.

 

METHOD:


RoboSep


RoboSep offers walk-away automation of immunomagnetic cell isolation by performing all EasySep cell labeling and magnetic separation steps. Set-up is simple: just load your samples and reagents and return to separated cells in as little as 25 minutes.

  • High Throughput
    Labels and separates up to 4 samples simultaneously.
  • Versatile
    Positive or negative selection of virtually any cell type from any species or cell source, including whole blood.
  • Fast and Efficient
    Cells are immediately available for flow cytometry, functional studies, or downstream analysis.
  • No magnetic separation columns
  • Capacity
    positive selection: 4 samples of up to 2x109 cells each;
    negative selection: 2 samples of up to 108 cells each.
  • Input volume
    from 0.25 ml to 8.5 ml.
  • Compatible -perfect companion for flow cytometry, no MicroBead removal required for further experiments.

Cell isolation kits for use with RoboSep.

Technical specifications for RoboSep.

To view a
comparison of magnetic beads and instruments from different suppliers, published by Biocompare in May 2009, go here.

Frequencies of Cell Types chart published by StemCell Technologies.