10-color Gallios (342W Borwell)




Optical Bench

Before you decide on a fluorochrome for a flow cytometric assay, you need to know:
  • the wavelengths of the lasers in the cytometer
  • the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer
  • the absorption and fluorescence wavelengths of fluorochromes to be used
These can be simulated using the BD fluorescence spectrum viewer

Violet laser configuration:


Blue laser configuration:




Red laser configuration: