Immune Monitoring Laboratory at Dartmouth-Hitchcock Medical Center


CMS - 1.6.8 - Pouebo

10-color Gallios (342W Borwell)

Before you decide on a fluorochrome for a flow cytometric assay, you need to know:

  • the wavelengths of the lasers in the cytometer
  • the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer
  • the absorption and fluorescence wavelengths of fluorochromes to be used
These can be simulated using the BD fluorescence spectrum viewer


Gallios Optical Bench

Violet laser configuration:

Blue laser configuration:


Red laser configuration:

Gallios red laser filters


Gallios video from Beckman Coulter


Stain index

Click here for Gallios Specifications.