Immune Monitoring Laboratory at Dartmouth-Hitchcock Medical Center

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CMS - 1.6.8 - Pouebo

Sector Imager 2400 (Meso Scale Discovery)

Description

The Sector Imager 2400 is a microplate detection platform built around a charge-coupled device (CCD) camera.  Electrodes are built directly into microplates to apply an electric potential to electrochemiluminescence (ECL) labels, stimulating luminescence signals on analytes.  This technique has high sensitivity due to a lack of background noise in the production of the chemiluminescent signal, hence is well suited to measuring analytes in serum and plasma.

References

Chowdhury F, Williams A, Johnson P. Validation and comparison of two multiplex
technologies, Luminex and Mesoscale Discovery, for human cytokine profiling. J
Immunol Methods. 2009;340:55-64. PubMed PMID: 18983846.

Dabitao D, Margolick JB, Lopez J, Bream JH. Multiplex measurement of proinflammatory cytokines in human serum: comparison of the Meso Scale Discovery electrochemiluminescence assay and the Cytometric Bead Array. J Immunol Methods. 2011;372:71-7. PubMed PMID: 21781970.

Tighe P, Negm O, Todd I, Fairclough L. Utility, reliability and
reproducibility of immunoassay multiplex kits. Methods. 2013 61:23-9.
PubMed PMID: 23333412.


System Components

  • Single and multiplex (up to 10-plex per well) plate formats
  • 96- and 384-well plates
  • Highly sensitive imaging detection systems
  • Rapid read times (reads a plate in just over 3 minutes)
  • Simultaneous reading of bar code labels on both short and long sides of microplates
  • No complicated fluidics
  • Six-log dynamic range

Advantages

  • Electrical stimulation gives rise to light output, thus removing issues of auto- fluorescence and other optical issues associated with using light for excitation of a reporter conjugate.
  • The localized nature of ECL around the electrode surface allows for wash-free detection procedures, as only surface captured reporter will respond to stimulation.
Disadvantages
  • Cost of requiring preprinted microtitre plates with proprietary electrode layers built into each well.
  • Currently available multiplexing assays using this platform extend to 10 analytes per well of a 96 well plate.

 

Sector Imager 2400 manual

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V-plex validated cytokine/chemokine assay kits menu