The mission of DartLab: Immunoassays and Flow Cytometry is to provide a broad array of services, instrumentation, support, education, and consultation to clinicians and basic scientists, especially in the area of translational research. We provide a wide variety of human immunology and mouse immunology services. These include up to 10-color cell subset phenotyping and intracellular staining, CFSE-lymphoproliferation assays, ELISPOTs, and multiplexed cytokine determination. We also provide operator-assisted cell sorting (FACS) and round-the-clock access to a variety of flow cytometers, from analog instruments to state-of-the-art digital multi-laser instruments. In addition, our services are available to Pharma for clinical trial end-point assays on a fee-for-service basis. Quality control is a priority of our Shared Resource as is Proficiency Testing of our Technologists.DartLab staff are available for consultation and assistance with planning, execution, analysis and interpretation of the immunological assays and the multi-color flow cytometry. We encourage users to become self-sufficient and have an active teaching and training program, including a 5-lecture flow cytometry course taught by the Director.
The Flow Cytometry Laboratory was established as a service core in Dartmouth Medical School in 1981 and became a Norris Cotton Cancer Center (NCCC) core in 1998. The Immune Monitoring Laboratory was established by Dr Channon Smith in 2002 with funding obtained by Drs Lloyd Kasper and Randy Noelle, together with COBRE and Cancer Center funding, specifically to carry out correlative immunological endpoint assays and mechanistic studies for clinical trials in addition to basic immunology research at Dartmouth Hitchcock Medical Center (DHMC). In 2010 the two laboratories were amalgamated following the retirement of the Flow Cytometry Laboratory director, Alice Givan. The consolidated Shared Resource moves beyond the simple provisioning of technical services to making complex experiments possible by providing scientific and technological support for implementing advanced, high-complexity assays in a cost-effective way, and by providing a mechanism for the acquisition of new methodologies. This Shared Resource is unique, not only at Dartmouth, but also regionally.
The Shared Resource is run under the overall guidance of an Advisory Board:
Marc Ernstoff, M.D.
Lloyd Kasper, M.D.
Craig Tomlinson, Ph.D.
Joyce DeLeo, Ph.D.
The Shared Resource is available for use 24 hours a day, 7 days a week. However, different rules apply to "normal hours" and "other hours." "Normal hours" are 9:00am to 5:00pm, Monday through Friday. If you use the instruments during "normal hours," you can expect them to be warmed up and QC'd. If you want to use the instruments during "other hours," you need to be aware of our rules and restrictions.
The Immune Monitoring Shared Resource is located on the seventh floor of the Rubin Building in the NCCC at DHMC in Lebanon, NH (710 Rubin). The Flow Cytometry Shared Resource is located in a suite of rooms on the third floor of the Borwell Building at DHMC (with an annex for one flow cytometer on the second floor of the Remsen Building (room 541) on the Hanover campus).