Immune Monitoring Laboratory at Dartmouth-Hitchcock Medical Center


CMS - 1.6.8 - Pouebo

8-color MACSQuant VYB (243 Remsen)

Optical bench

Before you decide on a fluorochrome for a flow cytometric assay, you need to know:
  • the wavelengths of the lasers in the cytometer
  • the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer
  • the absorption and fluorescence wavelengths of fluorochromes to be used

These can be simulated using the BD fluorescence spectrum viewer

Violet laser configuration:

MQ-VYB violet laser filters MQ-VYB violet laser fluor spectra


Blue laser configuration:

MQ-VYB blue laser filters MQ-VYB blue laser fluor spectra

Yellow laser configuration:

MQ-VYB yellow laser filters

MQ-VYB yellow laser fluor spectra


MACSQuant-VYB Specifications

Click here for MACSQuant VYB Specifications