Immune Monitoring Laboratory at Dartmouth-Hitchcock Medical Center

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CMS - 1.6.8 - Pouebo

8-color MACSQuant-10 (342W Borwell)

Optical Bench

Before you decide on a fluorochrome for a flow cytometric assay, you need to know:
  • the wavelengths of the lasers in the cytometer
  • the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer
  • the absorption and fluorescence wavelengths of fluorochromes to be used

These can be simulated using the BD fluorescence spectrum viewer

Violet laser configuration:

MQ-10 violet laser filters

MQ violet fluors emission spectra

 

 

Blue laser configuration:

MQ-10 blue laser filters

 

Red laser configuration:

MQ-10 red laser filters

MQ-10 red laser fluor emission spectra

 

Acquisition Request Form

Download 8-color MACSQuant 10 Acquisition Request Form here.

 

MACSQuant-10 Specifications

Click here for MACSQuant Specifications