Model Cell Lines for CF Research

Below is a list of cell lines available through the CF Cell Biology Core Facility.

*These cell lines are only available to Dartmouth Medical School researchers*
 

Pancreas

Name*
Origin
Genotype
Tight Junctions
CFPAC human pancreas dF508 Yes#
CFPAC + wt-CFTR  human pancreas dF508 + wt-CFTR Yes#

Airway

Name*
Origin
Genotype
Tight Junction
56FHTE8o- human trachea wt-CFTR No
9HTE0-  human trachea wt-CFTR No
5CFHTE8o human trachea dF-508/dF508 No
CFT-1 human trachea dF-508/dF508 Yes
CFT-1 + wt CFTR human trachea corrected Yes
CFTE-29o-  human trachea dF-508/dF508 No
IB3-1 human brochi dF508/1282X No
IB3-1 + wt-CFTR human brochi dF508/1282X + wt-CFTR No#
CFBE41o- human brochi dF508/dF508 ?
16HBEo14- human brochi wt-CFTR Yes
HBE-1 human brochi wt-CFTR ?
Calu-3 human submucosal tracheal epithelial cells (serous cells?) wt-CFTR Yes
2CFSMEo- human submucosal tracheal epithelial cells dF508/? No
2CFNPE9o- human nasal polyps dF508/dF508 No

 

Intestine

Name*
Origin
Genotype
Tight Junctions
HT-29
HT29.c19A
human colon wt-CFTR (low levels) Yes
T-84 human colon wt-CFTR (high levels) Yes

 

Kidney

Name*
Origin
Genotype
Tight Junctions
MDCK dog kidney wt-CFTR (low levels) Yes
MDCK GFP-CFTR dog kidney stable expression of GFP-wt-CFTR Yes
MDCK GFP-dF508 CFTR dog kidney stable expression of GFP-dF508-CFTR Yes
MDCK GFP-dTNR CFTR dog kidney stable expression of GFP-half-CFTR Yes
LLCPK1 pig kidney no CFTR  Yes
LLC-PK1 pig kidney stable expression of wt-CFTR Yes
LLC-PK1 pig kidney stable expression of dF508-CFTR Yes

*Cell lines in adjacent rows are segment matched or represent the CF line complemented with wt-CFTR.
#Divides slowly.
 
 

There is a long list of non-epithelial cells stably expressing wt-CFTR or dF508 CFTR including:
mammary tumor cells (C127) and fibroblasts, to mention but a few.
 

All lines are available in the CF Cell Culture Core Facility, 605 Remsen.
*These cell lines are only available to Dartmouth Medical School researchers*


 


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