The ATPase domain of TIP49 is required

for oncogenic transformation by c-Myc

A.

B.

REF assay

35

30

TIP49

25

20

15

10

TIP49

(D302N)

5

Walker A

Walker B

(aa70-77)

(aa302-305)

colony growth assay

TIP49 has WalkerA/WalkerB homology domains that

provide ATPase activity. Mutation of the WalkerB

motif (TIP49D302) creates an ATPase-deficient

protein that can still bind to TIP48 and c-Myc (A).

Oncogenic transformation by combined c-myc

and H-ras oncogenes is strongly suppressed by

cotransfection of the TIP49D302N mutant (B). The

TIP49D302N mutant does not suppress colony

formation with RSV-neo, indicating that the

protein is not inherently toxic to cells.

See Wood et al. Mol. Cell for details.

10

8

6

4

2

-