NEW CONFOCAL MICROSCOPE SYSTEM FOR THE ENGLERT LAB


We received an NIH Shared Instrumentation Grant and have purchased a new confocal microscope system for the Englert Cell Analysis Laboratory. It was set up in June, 2003, and is now being used extensively.

Specifications:

A Zeiss LSM 510 Meta Laser Scanning Confocal Microscope System:

Laser excitation lines: 405, 458, 477, 488, 514, 543, 633 nm

Photodetectors: two conventional photomultiplier tubes and one "meta" detector. The meta detector is a polychromatic multichannel detector which captures spectral information from the emitted fluorescence. With the meta detector, the instrument can store eight channels of fluorescence simultaneously (that is, cells can be imaged that have been stained with eight different fluorochromes). In addition, because the meta detector captures spectral information, fluorochromes with closely similar spectra (like fluorescein and GFP) can be distinguished.

Microscope: an inverted Zeiss Axiovert 200

Accessories: X/Y motorized stage; heated stage and heated objectives for live cell imaging