•the wavelength(s) of the laser(s) in the cytometer
•the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer
•the absorption and fluorescence wavelengths of possible fluorochromes
EXAMPLES OF SOME FLUOROCHROME POSSIBILITIES FOR THE FLOW CYTOMETERS
Before
you decide on a fluorochrome for a flow cytometric assay, you need to know:
•the wavelength(s) of the laser(s) in the cytometer
•the wavelengths of the filters in front of the photomultiplier tubes (PMTs)
in the cytometer
•the absorption and fluorescence wavelengths of possible fluorochromes