July 27 - August 1, 2003
Norris Cotton Cancer Center
Dartmouth Medical School
Dartmouth-Hitchcock Medical Center
Lebanon, New Hampshire, USA
The Sixteenth Annual Clinical Course on Flow Cytometry will
be held this year at Dartmouth-Hitchcock Medical Center in New Hampshire , USA
. As in past years, the Course will include hands-on laboratory and computer
work in small groups led by expert faculty. Lectures will cover a variety of
general and advanced topics and there will be many opportunities for informal
discussion. Emphasis of the Course will be divided between basic flow theory,
current practice in the clinical laboratory, and frontiers for the future. The
Course will be limited to approximately 45 participants who will rotate in groups
of about eight students through seven laboratory modules. Participants in the
Course will gain an understanding of the principles of flow cytometry and up-to-date
knowledge of the ways in which cytometry can be applied to, among others, the
following problems:
• leukemia and lymphoma phenotyping;
• analysis of platelets;
• analysis for cell cycle stage and ploidy;
• quality control and standardization;
• reference and alternative methods for CD4 measurements;
• CD34 stem cell quantification;
• selection of fluorochromes;
• staining of leukocytes for tetramers to evaluate antigen specificity;
• use of tracking dyes to follow cell proliferation;
• granulocyte function;
• analysis of specimens for microbiology.
Dartmouth-Hitchcock Medical Center , including both Dartmouth Medical School
and the Mary Hitchcock Memorial Hospital, is located on a new campus in Lebanon
, New Hampshire , a rural New England town on the Connecticut River . Lectures
and laboratories will be held at the Medical Center . Accommodations and social
events will make use of the neighboring facilities of historic Dartmouth College
. Transportation between events will be provided.
PROGRAM: Registration for the Course will
begin at 8:30 a.m. on Sunday, July 27th. Following registration, we will start,
at 10:00 a.m. , a series of lectures covering the principles and practice of
cytometric analysis. This all-day session will be followed by an informal reception
and dinner. The schedule for the rest of the week will include morning and afternoon
lectures on specific topics, each followed by 3-4 hour blocks of hands-on laboratory
and computer work. Participants will rotate through each of the seven practical
modules. Evenings will provide time for question periods, panel discussions,
and informal interactions as well as a guest lecture by Dr. Alan Waggoner. Tuesday
afternoon will be free for hiking, swimming, boating, and relaxing. On Thursday
evening, there will be a celebratory lobster bake. The Course will finish on
Friday morning, August 1st, after a closing lecture by Dr. Howard Shapiro.
FACULTY:
from Dartmouth Medical School:
ALICE GIVAN, PhD, Department of Physiology , Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
NORMAN LEVY, MD, Department of Pathology, Clinical Flow Cytometry Laboratory
PAUL WALLACE, PhD, Formerly, Department of Immunology and Microbiology ; Currently,
Roswell Park Cancer Institute, Buffalo , New York
guest faculty includes:
KENNETH AULT, MD, Maine Medical Center Research Institute, South Portland ,
Maine
BRUCE BAGWELL, MD, PhD ,Verity Software House, Topsham , Maine
NADEGE BERCOVICI, PhD, IDM Research Laboratory, Paris ,France
BRUCE DAVIS, MD, Maine Medical Center Research Institute, Scarborough, Maine
MARC GOLIGHTLY, PhD ,State University of New York , Stony Brook , New York
JAMES JETT, PhD, Los Alamos National Laboratory, Los Alamos , New Mexico
FRANK MANDY, Ph.D, Health Canada, Ottawa, Canada
JOHN MARTIN, PhD, Los Alamos National Laboratory, Los Alamos , New Mexico
KATHARINE MUIRHEAD, PhD, SciGro, Inc., Malvern , Pennsylvania
ABRAHAM SCHWARTZ, PhD, Center for Quantitative Cytometry, San Juan, Puerto Rico
HOWARD SHAPIRO, MD , Howard Shapiro, MD, PC, West Newton ,Massachusetts
CARLETON STEWART, PhD, Roswell Park Cancer Institute, Buffalo , New York
MARK WILDER, MS, Los Alamos National Laboratory, Los Alamos , New Mexico
ALAN WAGGONER, PhD, Carnegie Mellon University , Pittsburgh , Pennsylvania
INFORMATION:
DATES/TIME : Most participants will arrive on Saturday evening, July 26th. The
Course begins at 10:00 a.m. , Sunday, July 27th, and concludes about noon ,
Friday, August 1st.
PLACE : Lectures and laboratories will be held at the Dartmouth-Hitchcock Medical
Center, Lebanon, New Hampshire , USA .
AUDIENCE : The Course is directed at individuals with some experience in flow
cytometry. The Course is limited to approximately 45 participants.
ACCOMMODATIONS : Rooms ($44 per night, single) have been reserved for Course
participants in a modern, air conditioned dormitory at Dartmouth College in
Hanover , NH; 4-5 single rooms surround a shared bathroom/toilet/living area.
These rooms must be booked and paid for via the registration form. Other lodging
is available in the Lebanon area. Information will be sent on request. For participants
staying in the College dormitory, students in the past have suggested that we
tell you to bring your own soap, towel, and alarm clock.
ACCREDITATION : The Dartmouth-Hitchcock Medical Center designates this educational
activity for a maximum of 40 category 1 credits toward the AMA Physician's Recognition
Award. Each physician should claim only those credits that he/she actually spent
in the activity .
TRAVEL : Dartmouth-Hitchcock Medical Center is served by the Lebanon , NH airport
(10 minutes driving time), Manchester , N H airport (1.3 hrs.), Burlington ,VT
airport (2 hrs.), and Logan airport in Boston , MA (2.5 hrs.). Ground transportation
serves most of these airports. Complete travel information will be sent upon
registration. Off-site parking is available at some distance from the dormitory.
If you are staying in the Dartmouth dormitory, cars will not be required during
the week of the course. Shuttle busses will be available. For those wishing
to stay in other lodging, you will need a car to travel between your hotel and
the Medical Center . Parking near the Medical Center can be difficult.
COURSE REGISTRATION FEE : $1,200 (increasing to $1,300 after April 1). The fee
includes course materials, refreshment breaks, 6 breakfasts, 5 lunches, and
5 dinners (including reception dinner and lobster bake).
REFUNDS : A $50 fee will be retained on all cancellations made before June 1.
On June 1 or thereafter, half the fee will be retained. No refunds will be given
after June 30, 2003 on tuition or accommodation.
FURTHER INFORMATION : For registration or course information, or if you have
any special needs , contact the Center for Continuing Education in the Health
Sciences, Dartmouth-Hitchcock Medical Center, One Medical Center Drive, Lebanon,
New Hampshire 03756-0001 - Telephone 603-653-1524 or by e-mail to Maybe.I.Martinez@hitchcock.org.
Click to obtain REGISTRATION FORM.
Illustration: The distribution of tristetraprolin in a human
neutrophil after 2 hours of lipopolysaccharide stimulation. Tristetraprolin,
a TNFa mRNA regulatory protein, has been labelled
green with fluorescein. DNA in the nucleus has been counterstained red with
propidium iodide. Image courtesy of Anna-Marie Fairhurst and Kenneth Orndorff
in the Englert Cell Analysis Laboratory, Norris Cotton Cancer Center, Dartmouth-Hitchcock
Medical Center.