SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital analysis. Because SAGE does not require a preexisting clone, it can be used to identify and quantitate new genes as well as known genes. SAGE is from Johns Hopkins University.
Three principles underlie the SAGE methodology: 1) A short sequence tag (10-14bp) contains sufficient information to uniquely identify a transcript provided that that the tag is obtained from a unique position within each transcript; 2) Sequence tags can be linked together to from long serial molecules that can be cloned and sequenced; and 3) Quantitation of the number of times a particular tag is observed provides the expression level of the corresponding transcript.
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Connect to: SAGE - Serial Analysis of Gene Expression