Biology 23 Syllabus 2001

 

Lecture / Exam Schedule - Summer 2001

Lecture number

Date

Lecturer

Topic

Associated Chapters

1

June 21 - Th

TJ

Introduction, Protein and DNA Structure

1

2

June 26 - Tu

TJ

Bacterial Transcription I

9

3

June 27 - W

X-hour

TJ

Bacterial Gene Control I - Lac

10

4

June 28 - Th

TJ

Bacterial Gene Control I - Trp, Catabolite Repression

10

5

July 3 - Tu

TJ

Bacterial Gene Control II - Bacteriophage Lambda

11

July 4 - W

Independence Day Holiday

July 5 - Th

TJ

Exam #1 lectures 1-4 in class

 

 

6

July 10 - Tu

SB

Protein Synthesis I

5, 6

7

July 11 - W

X-hour

SB

Protein Synthesis II

7

8

July 12 - Th

SB

Chromatin Structure

19

9

July 17 - Tu

SB

Eukaryotic Transcription

20

July 18 - W

X-hour

SB/TJ

Review session - during X-hour

Exam #2 lectures 5-8 7-9 PM

10

July 19 - Th

SB

Eukaryotic Gene Control

21

11

July 24 - Tu

TJ

RNA Processing

22, 23

July 25 - W

X-hour

TJ

Lab overview

Lab manual

12

July 26 - Th

TJ

DNA Replication

12, 13

July 30 - M

Lab report #1 due at noon

13

July 31 - Tu

TJ

Mutagenesis / DNA Repair

14

14

August 1 - W

X-hour

TJ

DNA Repair

14

15

August 2 - Th

SB

Recombination / Retroviruses / Transposons

15, 16

16

August 7 - Tu

BG

Computational Biology -- lecture notes

Computational Biology - download powerpoint presentation

Bob Gross - guest lecturer

handout

August 8 - W

X-hour

SB/TJ

Review session during X-hour

Exam #3 lectures 9-15 7-9 PM

17

August 9 - Th

TJ

Generation of Immune Diversity

24

18

August 14 - Tu

SB

Signal Transduction

26

19

August 16 - Th

TJ

Molecular Aspects of Development

29

August 20 - M

Lab report #2 due at noon

20

August 21 - Tu

SB

Cell Cycle / Oncogenes

27, 28

August 23-25

TJ/SB

Review Session for Final , TBA

Final Exam lectures 16-20

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Professors

Prof. Tom Jack, 112 Gilman

Office Hours - 4-5 PM Wednesday (except 7/11) - If this time is not convenient, feel free to blitz and set up another time.

 

Prof. Sharon Bickel, 312 Gilman

Office Hours - 4-5 PM Monday (except 7/23) - If this time is not convenient, feel free to blitz and set up another time.

 

Lectures

Regular classes and X-hours will be held in 006 Steele.

 

Graduate Student TAs

Hayley Webber - Tuesday afternoon section

John Connolly - Tuesday evening section

Yingzhen Yang - Thursday afternoon section

Dhanalakshmi Nair - Thursday evening section

The TAs are most easily contacted via Blitzmail

 

Laboratory Coordinators

Ann Lavanway and Hazel Scott - 213 Gilman

 

Grading

First Exam (July 5) 15%

Second Exam (July 18) 15%

Third Exam (August 8) 20%

Final Exam 25%

Lab 25%

The median grade in this course the past several years has been a B. If there are a large number of either outstanding performances or very poor performances this summer then the median will be different and will reflect the overall performance of the class.

You are responsible for material that is covered in class. Most, but not all, of the lecture material is also in the textbook. Note that not everything in the chapters listed in the lecture schedule will be covered in lecture.

The topics and dates listed in the syllabus are approximate. Some of the topics might be covered in less than one lecture period, while others might take more. Think of the chapters as guides to what will be covered in class and as a reference to class notes.

 

Textbook

The textbook for the course is Genes VII by Benjamin Lewin. The textbook is available both at the Dartmouth Bookstore and at Wheelock Books.

Many of the figures and text from Genes VII are available at Ben Lewin's "Ergito" web site: http://www.ergito.com/. The web site contains animations and citations that are not in the textbook.

A laboratory manual will be handed out during the first week of lab. The lab manual will also be posted on the Bio 23 web site.

 

Lab Sections

Please disregard the section assignments made by the Registrar's office. You must complete a lab section assignment form to be assigned to a lab section.

You have four lab sections from which to choose:

Tuesday 2-6 PM and 7-11 PM

Thursday 2-6 PM and 7-11 PM.

Please fill out and return the lab section request form to 213 Gilman by 12:00 PM on Friday June 22. We will let you know your section assignment via blitz, hopefully by 10 AM on Monday, June 25.

The lab meets for the first time on Tuesday, June 26 and Thursday, June 28.

The lab meets in 208 Remsen. Lab lectures will be held in 216 Remsen.

Attendance at all labs is required for completion of the course.

 

Web Site

We have set up a web site for the course. The site will contain class lecture notes and other useful items. The URL is: http://www.dartmouth.edu/~bio23/

 

Lab Grading

The lab is worth 25% of the overall grade, and is broken down as follows:

Lab report #1 8%

Lab report #2 12%

Pre-lab questions 5%

 

Note to Students with Physical or Learning Disabilities:

We encourage students with documented disabilities, including "invisible" disabilities like chronic diseases and learning disabilities, to discuss with one of the professors appropriate accommodations that might be helpful. Please contact Prof. Jack or Prof. Bickel during the first week of class so that we have the time to implement any accommodations. All discussions will remain confidential.

 

Academic Honor Principle

The Dartmouth College Student Handbook states "Fundamental to the principle of independent learning are the requirements of honesty and integrity in the performance of academic assignments, both in the classroom and outside. Dartmouth operates on the principle of academic honor, without proctoring of examinations. Students who submit work which is not their own or who commit other acts of academic dishonesty forfeit the opportunity to continue at Dartmouth." There are a number of situations in which a student in Biology 23 might find him or herself tempted to violate the Academic Honor Principle. These situations include (but are not limited to) the following:

a) Examinations must be completed without reference to written materials other than those provided with the exam paper and must be completed without communication with anyone else (the only permissible exception is that students may request clarification of any exam question from the course faculty and staff who are present expressly for that purpose). The answers that you provide must be entirely your own work.

b) Under certain circumstances, we may allow exams to be re-graded by the instructors. Any alteration of the answers between the time when the graded papers were returned to the student and the time when the paper was submitted for re-grading constitutes a breach of the Academic Honor Principle. To deter this practice, we photocopy exam pages.

c) Laboratory experiments are performed in pairs, and we encourage collaborative analysis of the data. However, any report submitted for grading must represent the original words of the student submitting that report. Do not share computer files of work (including text, graphs, tables, etc.) to be submitted for grading! The student misrepresenting the work of another as his or her own is in violation of the Academic Honor Principle and it is quite possible that the Committee on Standards might find the student providing the original file also to be in violation.

Honesty is the foundation of the academic pursuit of knowledge. In recognition of this, the faculty and staff of Biology 23 will not overlook any violations of the Academic Honor Principle. Indeed, the Faculty Handbook of Dartmouth College states explicitly that College Faculty is obligated to report potential violations of the Academic Honor Principle to the Dartmouth College Committee on Standards.

 

Lab Schedule

June 26 and June 28 - Lab #1

Yeast two-hybrid, plating the mating

 

July 3 and 5- Lab #2

ß-galactosidase activity assays

 

July 10 and 12 - Lab #3

"Smash and grab"

Transformation of E. coli with shuttle plasmids

 

July 17 and 19 - Lab #4

Colony PCR

Agarose gel electrophoresis

 

July 25 - Lab Overview Lecture - Wednesday, July 25 during X hour (3-4 PM)

 

July 24 and 26 - Lab #5

Mini-preparation of plasmid DNA

PCR

Restriction enzyme digest

 

July 30 - Lab report #1 is due at noon Monday, July 30

 

July 31 and August 2 - Lab #6

Sequencing of plasmid DNA

DNA fragment isolation and elution

Ligation

 

August 7 and 9 - Lab #7

Transformation of ligations into electrocompetent E. coli

 

August 14 and 16 - Lab #8

Colony PCR

 

August 20 - Lab report #2 is due at noon Monday, August 20

 

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