Worm Protocols

Equipment

• Building a Fluorescent Dissecting Stereo-Microscope: Ambros Lab
• Avery Lab
  • Laser Ablation System
  • Simple EPG Rig

Microinjections

• Integrating extrachromasomal arrays: Michael Koelle
• Microinjecting worms: Michael Koelle
• Preparing for Injections: MGH

Stainings

• In situ hybridization: Geraldine Seydoux and Andy Fire
• Phalloidin Staining: Michael Koelle
• Dye Filling to Stain Amphid and Phasmid Neurons: Michael Koelle
• Staining C. elegans for B-galactosidase: Michael Koelle
• Antibody Staining: MGH

Dauer Protocols

• Dauer Pheromone Preparation: MGH
• Making Dauers with Pheromone: MGH

General

• Development of techniques for primary culture of C. elegans embryonic neurons Laird Bloom
  • Part 1
  • Part 2
  • Part 3
• Worm Codon Preferences: Ambros
• Floating Worms on Sucrose: MGH
• Cleaning Worm Stocks: Michael Koelle
• Freezing Worms: Michael Koelle
• Liquid Culture of Worms: Zhou
• Liquid culture of worms: Michael Koelle
• Novel soluble-compound chemotaxis assay Stephen Wicks

Mutants

• Gene Knockout ProtocolsLeon Avery
• Integrating extrachromasomal arrays: Michael Koelle
• EMS mutagenesis: Michael Koelle
• F2 Mutant Screen - non "clonal" Michael Koelle
• Freezing Worms: Michael Koelle
• Tc1-insertional mutatgenesis Ronald Plasterk
• EMS-deletion mutagenesis Gert Jansen
• Tc1-insertion display method Henri van Leunen
• Mapping Mutants using STS Polymorphisms: Michael Koelle

Drugs

• Levamisole Test: MGH
• Buffers: MGH
• Fixation and Permeabilization: MGH

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