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Bioluminescence
is the light produced in certain organisms as a result of luciferase-mediated
oxidation reactions. The luciferase genes, e.g.,
the genes from luminous beetle and, in particular the luciferase
from Photinus pyralis,
are very useful luminescent reporter genes. Despite its utility as a
reporter, native luciferase is not generally useful
in many organisms, including the filamentous fungi, because of low expression
levels. Dartmouth researchers in collaboration with their
colleagues from the University of Minnesota have now produced a novel nucleic acid sequence encoding a
luciferase enzyme which has a >100 fold higher
level of in vivo expression in organisms with a bias for cytosine (C)
or guanine (G) in the third position of the codon
than has been achievable with native firefly luciferase
reporter genes. Improved expression should result in greater reliability and
convenience as a genetic reporter in various microbial systems including
filamentous fungi (e.g., Neurospora, Aspergillus, Phythophthora.),
Gram-positive bacteria (e.g., Arthrobacter,
Streptomyces, Mycobacterium, Pseudomonas),
trypanosomes, and select vertebrates. Such applications include the
detection, location and measurement of gene expression in filamentous fungi;
measurement of enzymes, intracellular signaling and other turnover reactions
in cells or fluids; DNA and RNA binding assays; and immunoassay and other
protein assays. This
technology is claimed in the published Patent Corporation Treaty Application
No. PCT/US2005/035325. We are seeking
an industrial partner interested in its commercialization. (Ref: J284) |
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«Technology Transfer Office : Sponsored Projects : Dartmouth College |
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Phone: (603) 646-3027 |
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Fax: (603) 646-3670 |
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